Jeonghwan Son
BME PhD Defense Presentation

Date: 2023-08-03
Time: 10:00 AM-11:00 AM
Location / Meeting Link: EBB CHOA Seminar Room (https://gatech.zoom.us/j/96391571320)

Committee Members:
Shu Jia, PhD (Advisor); Francisco Robles, PhD; SungJin Park, PhD; Wilbur Lam, MD/PhD; Young Jang, PhD


Title: Toward Systems Miniature Optical Microscopy: Instrumentation and Biomedical Applications

Abstract:
Optical microscopy has offered versatile formats of imaging techniques, accompanied by the rapid development of miniature and open-source approaches, revolutionizing various biomedical applications. However, the adoption and utilization of recent optical imaging systems still face challenges due to the limited accessibility and applicability, restricting the potential applications of advanced optical microscopy. Thus, this thesis focuses on providing instrumentation and applications of recent fluorescence microscopy with high portability, accessibility, and adaptable solution via miniature and open-source approaches. The specific aims involve (1) advancing miniaturized microscopy (miniscopy) for high-resolution imaging of in vivo tissues and neural activity visualization in freely behaving mice, providing real-time insights into tissue dynamics and neural patterns; (2) developing systems for simultaneous in situ cell imaging using array miniscopy on multi-well plates, facilitating the study of cellular behavior and interactions within their native environment; and (3) advancing high-throughput imaging flow cytometry through the implementation of portable light-sheet microscopy, enabling three-dimensional characterization of cellular populations. The thesis strives to foster the development and application of innovative optical microscopy with the ultimate goal of enhancing biomedical research capabilities and facilitating discoveries in the field.